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To construct the blank vectors for gene overexpression capable of affinity cell sorting, we inserted the coding sequence of GST-EGFP-GPI DAF or GST-EGFP-GPI CEAM7 marker into pcDNA3.1 in place of the neomycin resistance gene and obtained pcDNA3.1-GST DAF and pcDNA3.1-GST CEAM7 (Figure 6A)
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